ISOLATION AND CHARACTERIZATION OF A SURFACTANT-STABLE PROTEASE FROM HALOPHILIC BACTERIA Chromohalobacter Japonicus BK-AB18

The protease from Chromohalobacter japonicus BK-AB18 was produced by growing bacteria in a LB medium containing 5% casein and NaCl. crude partially purified three levels of ammonium sulfate concentration (ranges 0–70%, 70–75% 75–80%) the highest specific activity exhibited range 75–80%. enzyme has relative molecular weight 65 kDa. this fraction had following optimum conditions: 7.5% NaCl, pH 9.0 temperature 45°C. at enhanced addition Ca2+ ion, but its significantly inhibited EDTA, hence is included as metalloenzyme. Interestingly, increased when exposed to 0.01% 0.05% SDS, relatively stable solution up 10%. It thus demonstrated that C. potential source produce extracellular can be applied surfactant/detergent industry.